Polymerase Chain Reaction (PCR) Steps

Polymerase Chain answer (PCR) StepsWe owe the disco rattling of the polymerase chain response to Kary B Mullis in the year 1983. He was the actual index of PCR. Few people atomic number 18 aw atomic number 18 that in 1971, Kleppe and the Nobel laureate Gobind Khorana published studies including a description of techniques that ar now known to be the basis for nucleic acidulous replication. However, it is unfortunate that Kleppe and Khorana were ahead of their times. Oligonucleotide synthesis wasnt as simple as it is today genes had non been seasond and the idea of ther virtuallyable deoxyribonucleic acid polymerases had non been described. Hence, the credit for discovering the PCR remains with Kary Mullis.The Polymerase Chain Reaction is essentially a cell-free method of deoxyribonucleic acid and RNA cloning. The deoxyribonucleic acid or RNA is isolated from the cell and replicated upto a million times. At the end, what you shake up is a greatly amplified fragment of de oxyribonucleic acid. The PCR is quick, reliable and sensitive and its variations fork out made it the basis of genetic testing.WHAT KARY B MULLIS SAYS ABOUT HOW HE DISCOVERED THE POLYMERASE CHAIN chemical reactionI was just driving and thinking about ideas and suddenly I saw it. I saw the polymerase chain reaction as clear as if it were up on a blackboard inside my head, so I pulled over and started scribbling. A chemist friend of his was asleep in the car. Mullis says that Jennifer objected groggily to the delay and the light, unless I exclaimed I had discovered something fantastic. Unimpressed, she went back to sleep.Mullis kept scribbling calculations, right in that respect in the car. He convinced the small California biotech company, Cetus, he was working for at that time, that he was up to something big. They finally listened. They sold the patent of PCR to Hoffman-LaRoche for a staggering $300 million the maximum meter of money ever paid for a patent. Mullis meanwhile re ceived a $10,000 bonus.BASIS OF THE METHODThe purpose of PCR is to generate a huge number of copies of a segment of deoxyribonucleic acid, which could be a gene, a portion of a gene, or an intronic constituent. There argon tercet major steps in a PCR, which argon repeated for 30 or 40 cycles. This is done on an automated cycler, which bed either heat or cool the tubes containing the reaction variety show, as required, in a very short peak of time. There atomic number 18 three major steps in a PCR, which atomic number 18 repeated for 30 or 40 cycles.DenaturationDuring this process, the reiterate baseed DNA melts and opens to form single stranded DNA. every enzymatic reactions, such as those carried over from a previous cycle, stop. This will be explained in the next paragraph. The temperature for denaturation is non fixed but it usually buy the farms at about 95C. It is central to realize that the denaturation temperature is largely dependent on GC (guaninecytosine) con tent of the DNA fragment to be analyzed. This is reasonable when one considers that the GC bond is a triple hydrogen bond and the AT bond is a double bond. Logic dictates that a triple bond should be 1.5 times harder to break than a double bond. Therefore, when the segment of DNA to be analyzed has a very lavishly GC content, the denaturation temperature idler r to each one even upto 99C.AnnealingThis requires temperatures lower than those required for denaturation. In this process, the fuzees season to that very specific segment of DNA that is to be amplified. The basiss are jiggling around, ca employ by the Brownian motion. Ionic bonds are constantly formed and broken between the single stranded soil and the single stranded template. The more(prenominal) stable bonds last a little bit longer ( grounds that fit exactly) and on that little piece of what is now double stranded DNA (template and soil) the polymerase mountain attach and starts copying the template. Once the re are a few bases built in, the bonce bond is so strong between the template and the fuzee, that it does not break anymore.ExtensionThis is done at 72C. This is the ideal temperature for working with polymerase. The primers, which are complementary to the template, already have a strong ionic attraction to the template. This military force is stronger than the forces breaking these attractions i.e. the luxuriously temperature. Primers that are on positions with no exact match (non complementary) get loose again (because of the last temperature) and dont give an extension of the fragment. The nucleotide bases are added from the 5 end to the 3 end. The phosphate group of the dNTPs is coupled with the hydroxyl group of the extending DNA strand. The extension time depends on 2 factors the type of polymerase employ and the length of the DNA fragment to be amplified. Usually, Taq polymerase adds dNTPs at the rate of about 1000 bases per minute.It is important to realize that each component of the PCR including the input DNA, the oligonucleotide primers, the thermostable polymerase, the buffer and the make pass parameters has a profound impact on the sensitivity, specificity and fidelity of the reaction.The three steps of the first cycle are shown, that is, denaturation, annealing and extension. At the end of the first cycle, two strands have been synthesized. At the end of the second cycle, four strands have been synthesized (the three steps of the cycle have not been shown). At the end of the third cycle, 8 strands have been synthesized. The number of strands increases exponentially with each cycle.NuggetsThe Polymerase Chain Reaction is essentially a cell-free method of cloning DNA and RNA.There are three steps involved in every cycle these are denaturation, annealing and extension.At the end of each cycle, the DNA doubles. Therefore, theoretically, if there are n cycles in a reaction, the number of DNA fragments at the end of the reaction will be 2n.CO MPONENTS OF THE POLYMERASE CHAIN REACTIONThe components that are essential for a successful PCR are elaborated here.TEMPLATE DNAThis is that portion of the DNA/gene that is to be amplified. Usually the concentration is100 ng genomic DNA per PCR reaction. However, this can vary depending on the target gene concentration and the source of DNA. The PCR reaction is inherently sensitive. It is not necessary for the template DNA to be abundant or highly purified. high amounts of template DNA can increase the yield of nonspecific PCR products, but if the fidelity of the reaction is crucial, one should limit both template DNA quantities as well as the number of PCR cycles.DNA in solution may contain a large number of contaminants. These contaminants may inhibit the PCR. few of these reagents are phenol, EDTA, and protease K, which can inhibit Taq DNA polymerase. However, isopropanol precipitation of DNA and washing of DNA pellets with 70% ethanol is usually effective in removing traces o f contaminants from the DNA sample.Effects of FixationThis is of particular interest to the pathologist since he has to deal with formalin fixed tissue. DNA extracted from fresh tissue or cell suspensions forms an optimal template for PCR. The tissue is best stored at -70C at which the nucleic acids can be stored indefinitely. A temperature of -20C is sufficient to preserve the DNA for several months and at 4C, the DNA can be stored for several weeks. At room temperature, the DNA has been successfully stored for hours to days however, mitochondrial DNA is very sensitive to temperature and may shame in thawed tissues.DNA extracted from fixed tissue has been used successfully for PCR. The type of fixative and the duration of fixation are of critical importance. Non crosslinking fixatives homogeneous ethanol grant the best DNA. Formaldehyde is variable in its DNA yield. Carnoys, Zenkers and Bouins are poor fixatives as far as DNA preservation is absorbed.Not surprisingly, formalde hyde is the fixative which has been evaluated the most, because it is more commonly used world round-eyed. The studies have demonstrated that a successful PCR depends on the protocol to extract the DNA and the length of fixation. Formaldehyde reacts with DNA and proteins to form reactive hydroxymethyl talk termss which give rise to a salmagundi of end products which include DNA-DNA and DNA-protein adducts. Purification of DNA from formalin fixed tissue, therefore, includes heating to reverse the hydroxymethyl additions and treatment with a proteinase to hydrolyze the covalently associate proteins. However, there is no way to reverse the DNA-DNA links and these links inhibit the DNA polymerases. This accounts for the low PCR yield which is seen with formalin fixed tissue. Usually, the PCR reaction with formalin fixed DNA as a template yields products which are not more than 600 bp in size.NuggetsTemplate DNA is required in a concentration of 100ng for each PCR reaction. Contamina nts in DNA may inhibit the reaction.Fixation of tissues brooks DNA which is not as good as DNA obtained from fresh/ polar tissues.Different fixatives give different DNA yields. Alcohol is the best fixative and Carnoys, Zenkers and Bouins are poor fixatives as far as DNA preservation is concerned. Formalin is intermediate in DNA yield.Purification of DNA from formalin fixed tissue involves heating to reverse the attachment of hydroxymethyl intermediates and treatment with a proteinase to hydrolyze the covalently linked proteins.The DNA obtained afterward fixation can be used for reactions in which the PCR product is not more than 600 bp.PCR BUFFERThe purpose of using buffers in PCR is to provide optimum pH and potassium ion concentration for the DNA polymerase enzyme (usually obtained from bacteria Thermus aquaticus) to function. Most buffers are available in a 10X concentration and require dilution before use. Although most protocols recommend the final buffer concentration of 1X , a concentration of 1.5X might result in increased PCR product yield.The PCR buffer contains many components. Some important ones are discussed hereDivalent and monovalent cations These are required by all thermostable DNA polymerases. Mg2+ is the divalent cation that is usually present in most of the PCR buffers. Some polymerases also work with buffers containing Mn2+. Calcium containing buffers are ineffective and therefore, rarely used. Buffers can be divided into first and second generation buffers on the basis of their ionic component. The second generation buffers, as opposed to first generation buffers, also contain (NH4)2SO4 and permit consistent PCR product yield and specificity over a wide range of magnesium concentration (1.0 to 4.0 mM MgCl2). The overall specificity and yield of PCR products is better with second generation buffers, as compared with first generation PCR buffers. Buffers also contain KCl. Salts identical KCl and NaCl may help to facilitate primer annea ling, but concentration of 50 mM will inhibit Taq polymerase activity. Interactions between K+ and NH4+ allow specific primer hybridization over a broad range of temperatures. Magnesium is one of the most important components of the buffer. Mg2+ ions form a soluble complex with dNTPs which is essential for dNTP internalisation they also stimulate polymerase activity and influence the annealing efficiency of primer to template DNA. The concentration of MgCl2 can have a dramatic effect on the specificity and yield of PCR products. Optimal concentration of MgCl2 is between 1.0 to 1.5 mM for most reactions. Low MgCl2 concentration helps to eliminate non-specific priming and formation of background PCR products. This is desirable when fidelity of DNA synthesis is critical. At the same time, however, too few Mg2+ ions can result in low yield of PCR products. High MgCl2 concentration helps to stabilize interaction of the primers with their mean template, but can also result in nonspecifi c binding and formation of non specific PCR products. It is important to be aware that many PCR buffers (often sold in 10X stocks) already contain some amount of MgCl2. Therefore, the addition of further amounts must be carefully monitored. In the best possible scenario, the PCR would work well with the amount of Mg2+ already present in the buffer solution. However, if this does not occur, it is necessary to standardize the amount of Mg2+ in the reaction mix. This can be difficult because the dNTPs and the oligonucleotide primers bind to Mg2+. Therefore, the milling machinery concentration of Mg2+ must exceed the molar concentration of the phosphate groups contributed by dNTPs and the primers. As a rule of thumb, the magnesium concentration in the reaction mixture is generally 0.5 to 2.5 mM greater than the concentration of dNTPs. The optimal concentration of Mg2+ should, therefore, be standardized for each reaction.Tris-Cl The concentration of tris-Cl is adjusted so that the pH o f the reaction mixture is represented between 8.3 and 8.8 at room temperature. In standard PCR reactions, it is usually present in a concentration of 10mM. When incubated at 72C which is the temperature for extension, the pH of the reaction mixture falls by more than a full unit, producing a buffer whose pH is 7.2.Other components Some buffers also contain components wish BSA (Bovine serum albumin) and DMSO (dimethyl sulphoxide). BSA reduces the amount of template sticking to the side of the tube, making it available for elaboration and reducing the risk of primer dimer. Primer dimers are products obtained when the primers anneal to each other instead to to the template DNA. DMSO has been shown to facilitate DNA strand separation (in GC rich difficult secondary structures) because it disrupts base pairing and has been shown to improve PCR efficiency.In effect, it is wise not to tamper with the buffer provided with the Taq polymerase. The buffer is usually standardized for the vi al of Taq and there is no need to add additional MgCl2 or stabilizers like DMSO and BSA. However, some Taq buffers come with the buffer in one vial and MgCl2 in a separate vial. Under such circumstances, it is wise to start with 1L of MgCl2 and increase its concentration in aliquots of 0.5 L, if the initial reaction fails.NuggetsThe PCR buffer contains divalent and monovalent cations, Tris Cl and other components.The PCR buffer is used to give the correct pH and potassium concentration for the DNA polymerase to function.The most common divalent ion used is magnesium in the form of MgCl2. MgCl2 concentration is vital for PCR.Tris Cl is used to maintain the pH between 8.3 and 8.8 at room temperature.Salts like NaCl and KCl may facilitate primer annealingOther components like BSA and DMSO help to increase the sensitivity and specificity of the reaction.OLIGONUCLEOTIDE PRIMERSWhat are Oligonucleotide Primers?PCR primers are short fragments of single stranded DNA (17-30 nucleotides in l ength) that are complementary to DNA durations that flank the target region of interest. The purpose of PCR primers is to provide a free 3-OH group to which the DNA polymerase can add dNTPs.There are two primers used in the reaction. The forward primer anneals to the DNA minus strand and directs synthesis in a 5 to 3 direction. The installment of primers is always represented in a 5 to a 3 direction. The reverse primer anneals to the other strand of the DNA.How to design a primer?The predominant goal kept in mind while designing a primer is specificity. apiece member of the primer must anneal in a stable fashion to its target sequence in the template DNA. The longer the primer, the high is its specificity. Unfortunately, the longer the primer, the less likely it is to anneal to a particular sequence in the template DNA. Conversely, if the primer length is small, it is likely to anneal, but its specificity will be poor. A compromise is reached by designing primers between 20 and 25 nucleotides long. Inclusion of less than 17 nucleotides often leads to non specific annealing, while presence of more than 25 nucleotides may not allow annealing to occur at all.Remember that the DNA sequence in the human genome appears to be a stochastic sequence of nucleotides. When designing primers, it is important to calculate the probability that a sequence exactly complementary to a string of nucleotides in the human genome will occur by chance. Several formulae are designed to calculate such probabilities. However, mathematical expressions are not necessarily correct and in this case, the predictions maybe wildly prostitute. The distribution of codons is non random with repetitive DNA sequences and gene families. It is advisable to use primers longer than the statistically indicated minimum. It is also advisable to scan DNA databases to tot if the proposed sequence occurs only in the desired gene.For a practicing pathologist, it is best not to attempt designing of prim ers. What a pathologist requires is the primer sequence for an established test. If, for example, a pathologist requires primer sequence for the diagnosis of sickle cell anemia, all he has to do is search the web for papers related to molecular testing of sickle cell anemia. The primer sequences will be provided in the paper. Custom made primers can be commercially synthesized. Several biotechnology companies provide this facility. to begin with the primers are ordered, it is essential to check that the sequence is correct and that there are no missing nucleotides in the sequence. That is where, pillory is invaluable.Before the intricacies of the BLAST search are elaborated upon, it is necessary to have in mind that designing a primer does not depend only on the sequence of nucleotides. Other factors like the GC content and melting point are also important considerations. They will be dealt with later in the chapter.BLAST and its usesBLAST is an acronym for Basic Local Alignment Search Tool. It is an algorithm comparing development about primary biological sequences with a library or database of sequences.A BLAST can be performed for different organisms, but in this book, we will concern ourselves with nucleotide BLAST in humans only. BLAST searches the database for sequences similar to the sequence of interest (the query sequence) by using a 2-step approach. The basic concept is that the higher the number of similar segments between two sequences, and the longer the length of similar segments, the less divergent the sequences are, and therefore, likely to be more genetically related (homologous).Before perfoming a BLAST search the oligonucleotide sequence is first identified. The sequence is fed into the programme. BLAST first searches for short regions of a given length called rowing (W). It whence searches for substrings which are compared to the query sequence. The program then aligns with sequences in the database (target sequences), using a substit ution matrix.For every pair of sequences (query and target) that have a word or words in common, BLAST extends the search in both directions to find alignments that score greater (are more similar) than a certain score threshold (S). These alignments are called high scoring pairs or HSPs the maximal scoring HSPs are called maximum segment pairs (MSPs).The BLAST search as outlined in fig 7.2 shows the results of the search. If we scroll batch further, we can see the sequences producing significant alignments. Note that in this BLAST search, there are 49 BLAST hits in the query sequence.In the list shown in figure 7.2, there is a list of hits starting with the best (most similar). To the right of the screen is the E-value. This is the expected number of chance alignments the lower the E value, the more significant the score. First in the list is the sequence finding itself, which obviously has the best score. To the left is the accession number. This refers to a unique code that ide ntifies a sequence in a database.It is important to know that there is no set cut-off that determines whether a match is significant or similar enough. This must be determined gibe to the goals of the project.The sequences provided in the figure 7.2 show a significant alignment with Pseudomonas japonica. It shows a high score (bits) and a low E-value. Note that the lower the E value, the greater the likelihood that the sequence is a good match.BLAST output can be delivered in a variety of formats. These formats include HTML, plain text edition and XML formatting. For the NCBIs web-page, the default format for output is HTML. When performing a BLAST on NCBI (National Centre for Biotechnology Information), the results are displayed in a graphical format present the followingThe hits foundA tabular form showing sequence identifiers for the hits with scoring related dataAlignments for the sequence of interest and the hits received with corresponding BLAST scores for these.The easiest to read and most informative of these is probably the table. The main idea of BLAST is that there are often high-scoring segment pairs (HSP) in a statistically significant alignment. BLAST searches for these high scoring sequence alignments between the query sequence and the sequences in the database. The speed and relatively good accuracy of BLAST are among the key technical innovations of the BLAST programs.Sequence of events to be followed when performing a BLAST search.Go to PUBMED (http//www.ncbi.nlm.nih.gov/pubmed/)Scroll down to reach a heading called best-selling(predicate)Under POPULAR click on BLASTClick on nucleotide blastUnder the heading, enter accession number(s), gi(s), or FASTA sequence(s), type or paste the sequence that you want matched.Click BLASTWait for the results. Analyse the nucleotide sequence as it appears.Calculation of Melting TemperatureThe melting temperature or Tm is a mea sure as shooting of stability of the duplex formed by the primer and the compl ementary target DNA sequence and is an important consideration in primer design. Tm corresponds to the midpoint in transition of DNA from the double stranded to its single stranded form. A higher Tm permits an increased annealing temperature that makes sure that the annealing between the target DNA and the primer is specific. The Tm is dependent on the length of the oligonucleotides and the G+C content of the primer. The formula for calculation of Tm is given in table 7.1.Table 7.1 Formula for calculation of the melting temperature.Length of PrimerTm (C)Less than 20 nucleotides long2(effective length*)20 to 35 nucleotides long22 + 1.46(effective length)*Effective length = 2(number of G+C) + number of (A + T)Primers are usually designed to stay off matching repetitive DNA sequences. This includes repeats of a single nucleotide.. The two primers in a PCR reaction are not homologous to each other and their complementarity can lead to formation of spurious amplification artifacts calle d primer dimers. The 3 end of a primer is most critical for initiating polymerization.The rules for selecting primers in addition to those already mentioned are as followsThe C and G nucleotides should be distributed uniformly throughout the primer and comprise approximately 40% of the bases. More than three G or C nucleotides at the 3-end of the primer should be avoided, as nonspecific priming may occur.The primer should be neither self-complementary nor complementary to any other primer in the reaction mixture, in order to avoid formation of primer-dimer or hairpin-like structure.All possible sites of complementarity between the primer and the template DNA should be noted.The melting temperature of flanking primers should not differ by more than 5C. Therefore, the G+C content and length must be chosen accordingly (a higher G+C content means a higher melting temperature).The PCR annealing temperature (TA) should be approximately 5C lower than the primer melting temperature.G+C cont ent in each primer should not be more than 60% to avoid formation of internal secondary structures and long stretches of any one base.Primer extension will occur during the annealing step. Primers are always present in an excess concentration in conventional (symmetric) PCR amplification and, typically, are within the range of 0.1M to 1M. It is generally advisable to use purified oligomers of the highest chemical integrity.Primer DimersA Primer Dimer (PD) consists of primer molecules that have attached or hybridized to each other because of strings of complementary bases in the primers. As a result, the DNA polymerase amplifies the PD, leading to competition for PCR reagents, thus potentially inhibiting amplification of the DNA sequence targeted for PCR amplification.In the first step of primer dimer formation, two primers anneal at their respective 3 ends. The DNA polymerase will bind and extend the primers. In the third step, a single strand of the product of step II is used as a template to which fresh primers anneal leading to synthesis of more PD product.Primer dimers may be visible after gel electrophoresis of the PCR product. In ethidium bromide stained gels, they are typically seen as 30-50 base-pair (bp) bands or smears of moderate to high intensity. They can be easily elevated from the band of the target sequence, which is typically longer than 50 bp.One approach to prevent PD formation consists of physical-chemical optimization of the PCR system, i.e., changing the concentration of primers, MgCl2, nucleotides, ionic aptitude and temperature of the reaction. Reducing PD formation may also result in reduced PCR efficiency. To overcome this limitation, other methods aim to reduce the formation of PDs only. These include primer design, and use of different PCR enzyme systems or reagents.NuggetsOligonucleotide primers are short fragments of single stranded DNA (17-30 nucleotides in length) that are complementary to DNA sequences that flank the target r egion of interest. They dictate which region of DNA in the PCR will be amplified.Primer sequences can be obtained by reviewing previously published literature. A confirmation of the sequence can be done by using BLAST (Basic Local Alignment Search Tool).The melting temperature is the midpoint in the observed transition from a double stranded to a single stranded form. A higher annealing temperature ensures that the annealing between the target DNA and the primer is specific.A primer dimer consists of primer molecules that have attached or hybridized to each other because of strings of complementary bases in the primers. Taq polymerase amplifies the primer dimer leading to competition for the PCR products.Several methods are used to reduce primer dimer formation including changing the concentrations of primers, MgCl2, nucleotides, ionic strength and temperature of the reaction.TAQ DNA POLYMERASEThe initial PCR reaction used the Klenow fragment of Escherichia coli DNA polymerase. Howe ver, this was unstable at high temperatures and it was necessary to add a fresh aliquot of enzyme after every denaturation step. The annealing and extension temperatures had to be kept low and as a result, there was formation of non specific products in abundance. The discovery of the thermostable Taq DNA polymerases ensured that the PCR did not remain a laboratory curiosity. The extension and annealing temperatures could now be kept high and the formation of non specific products was greatly reduced.Taq became famous for its use in the polymerase chain reaction and was called the Molecule of the Year by the journal Science.Why Taq?Taq is the enzyme of choice in PCR because of the following reasonsTaq works best at 75C80C, allowing the elongation step to occur at temperatures which make non-Watson-Crick base pairing a rare event.It can add upto 1,000 nucleoside triphosphates to a growing DNA strand.Taq has a half-life of 40 minutes at 95C and 9 minutes at 97.5C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72C.Because of all these properties, Taq is the enzyme of choice in the PCR.How does Taq polymerase act?The first requirement is a primer. The primer is annealed to the template strand having free hydroxyl group at its 3 end. During the extension phase, the Taq synthesizes a newfangled DNA strand complementary to the template by adding dNTPs in a 5 to 3 direction condensing the 5 phosphate group of the dNTPs with the 3 hydroxyl group of the end of the extending DNA strand. Since Taq works best between 70C- 80C, a temperature of 72C is usually chosen as the optimum annealing temperature.Where does Taq come from?In Thermus aquaticus, Taq polymerase is expressed at very low levels and commercial production is not economically viable. However, the enzyme can now be produced from different versions of the engineered Taq gene so as to obtain high levels of expression in E coli.What other polymerases are available for use in PCR?Taq is not the only polymerase other polymerases are available but Taq is the one that is generally used in a PCR. A few other polymerases with their uses are as followsPFU DNA polymerase -Found in Pyrococcus furiosus, it functions in vivo to replicate the organisms DNA. The main deviation between Pfu and alternative enzymes is the Pfus superior thermostability and proofreading properties compared to other thermostable polymerases. Unlike Taq DNA polymerase, Pfu DNA polymerase possesses 3 to 5 exonuclease proofreading activity, meaning that it works its way along the DNA from the 3 end to the 5 end and corrects nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. As a result, Pfu is more commonly used for molecular cloning of PCR fragments than the historically popular Taq. However, Pfu is slower and typically requires 1-2 minutes to amplify 1kb of DNA at 72 C. Pfu can also be used in conju nction with Taq polymerase to obtain the fidelity of Pfu with the speed of Taq polymerase activity.TFL DNA polymerase Obtained from Thermus flavus, it is useful for the amplification of large segments of DNA.WHAT IS FIDELITY?All DNA polymerases have an intrinsic error rate that is highly dependant on the buffer composition, pH of the buffer, dNTP concentration and the sequence of the template itself. The types of errors that are introduced are frameshift mutations, single base pair substitutions, and spontaneous rearrangements. Therefore, the PCR reaction generates a product that is very similar, but in many cases, not identical to the original sequence. The measurement of dissimilar product obtained is obviously related to the cycle in which the mismatch took place. Under normal circumstances, this does not make any difference however, these errors may plough significant during sequencing when the role of fidelity comes into play.Fidelity is the ability of the polymerases to avo id the incorporation of wrong nucleotides during the reaction. Under normal circumstances, it really does not make a difference if a wrong nucleotide is incorporated because the size of the PCR product remains the same and that is what we have to look for. However, there are some polymerases like Pfu which have a high fidelity. In addition to reading from the 5 to the 3 direction, they can also read from the 3 to the 5 direction and correct the wrong nucleotides wh